Wet-fixed smears are mainly stained by Papanicolaou (PAP) staining [] but some of them also by hematoxylin-eosin (H&E) staining.On the other hand, air-dried smears are stained with May-Grunwald-Giemsa (MGG), Giemsa [], or Wright staining [].MGG stain is also called Pappenheim stain in Germany . Alternatively, the prepared slide can be shipped (unstained or stained) to a. Epithelial cells seen during wet-mount examination are traditionally classified as squamous or nonsquamous. endobj In addition to immunocytochemistry, the samples are suitable for DNA extraction for genetic studies and in situ hybridisation (eg EBER for EBV and c-myc translocation) (Van Noorden et al 2011). Accessibility Moreover, it is argued that immediate fixation is superior because it kills the cells uniformly and suddenly; in addition, it protects the cells from the effects of putrefaction, degeneration, and autolysis and facilitates uniform cellular preservation and staining. Bethesda, MD 20894, Web Policies A new procedure for staining vaginal smears. HTP Flashcards | Quizlet Acta Cytol. Air-drying artifact was more frequent in AD smears compared to those of WF (p = 0.036) but was of only mild degree in all cases. HHS Vulnerability Disclosure, Help Vielh WP. Faculty of Medicine, Nara Hospital, Kindai University, Ikoma, Japan. The air-dried smears were also served as retained cellularity control. Accessibility The second method has the advantage of providing more material but the disadvantage of requiring transport in an alcoholic solution which can cause problems with couriers (but see below for storage and transport using the PEG solution). Because of price constraints, the preparation of cytospins and the Thinprep LBC method mentioned above may be out of reach of laboratories in developing countries, also the immunocytochemical tests which confirm and expand the original diagnosis made on clinical grounds together with Giemsa- or Papanicolaou-stained smears. 2 0 obj doi: 10.1002/brb3.805. One of the two wet-fixed smears was processed as a control of preservation of nuclear detail whereas the other one stayed air-dried for 10 minutes and then covered with normal saline (rehydration method) for 80 seconds before wet fixation. One is that advocated by the UKNEQAS for Immunocytochemistry and applies to smears, imprints or cytospin preparations. fixation of cytology specimens is critical to the preservation of the cellular components. x=ms63/;1M Avrs:$lowBRi, bOm}Q-mZGmnWowXf]5''g_IbQR& Rehydrated air-dried Pap smears as an alternative to wet-fixed smears. As part of a complete urinalysis, wet-mount samples are routinely reviewed unstained.
